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RRJOMS | Volume 5 | Issue 4 | July, 2017
July 27-29, 2017 Vancouver, Canada
10
th
International Conference on
Emerging Materials and Nanotechnology
Quantifying the biological fate of nanosilver
David Kennedy
National Research Council of Canada, Canada
W
ith a growing number of high precision tools for studying biological systems, it is important to develop traceable
quantitative methods that result in accurate measurements. Because biological systems are both complex and fluxional,
context is vitally important for such measurements in order for them to be accurate. Correlation of measurements through
space and time can provide such quantitative assessments. Metallic nanoparticles pose many challenges for measurement in
cellular systems. The metal can interfere with the detection method and the particles can change in size and shape over time
and in association with different biological molecules. At the National Research Council, we seek to correlate detailed physical
characterization of silver nanoparticles with biological measurements to generate methods for measuring the impact of
nanosilver on different cell types and quantifying the specific interactions of nanosilver with biological molecules. Correlating
changes in nanoparticles over time in biological fluids helps to provide an understanding of nanoparticle behaviour and results
in higher reproducibility of observed biological endpoints. Surface coatings play a pivotal role in recognition of the particles by
cellular receptors suggesting active transport plays a critical role in the nanosilver life cycle. Physical and chemical differences
between silver nanoparticles and changes that occur in biological test media can be correlated to toxicity, and different
mechanisms for toxicity are apparent. Uptake rates and localization is also different between different cell lines. Uptake and
localization of particles provides evidence that nanosilver should not be treated as a single material but should be studied as an
array of materials with different properties in different biological systems.
Biography
David Kennedy is an expert in Biological Inorganic Chemistry with nearly a decade of experience working at the nano-bio interface. He currently works in the areas
of nano- and bio-metrology at the National Research Council Canada. Previously, he has also held posts in Chemical Biology, Molecular Imaging and Nanomedicine
both at the NRC and MPI in Berlin, Germany. Currently, he is focused on building new tools for standardizing measurements of nanomaterials in biological systems.
This also includes the use of new nanobiomaterials used to mimic living tissues. Research in his lab also partners across several other government organizations
including Health Canada, Environment Canada and the Canadian Food Inspection Agency, as well as several different parts of the NRC.
David.Kennedy@nrc-cnrc.gc.caDavid Kennedy, Res. Rev. J Mat. Sci. 2017
DOI: 10.4172/2321-6212-C1-002