The effect of Silver nanoparticles [AgNPs] on chlorophyll A and Î²-carotene content [as two natural antioxidants] in the microalgae Chlorella vulgaris
Chlorophyll as a green pigment found in plants, algae and cyanobacteria, this biomolecule considered as the most important molecule in photosynthesis, which allows plants to absorb energy from light. In other hand β-carotene as a colored pigment with antioxidant activity currently is the most food additive. Beside one, metal nanoparticle [NPs], attracts an increasing interest, due to their new and different chemical, physical and biological characteristic as compared with those of macroscopic phase that allows attractive application in various fields. Chlorella vulgaris is a single-cell of green algae, which believe to have high potential in photosynthetic efficiency that could serve as food source. In the current study we have evaluated the arginine coated AgNPs and naked AgNPs in two independent groups on chlorella vulgaris chlorophyll and β-carotene content. The biologically synthesized AgNPs were provided and used in the concentration of 100, 200 and 400 μg/mL. Micro algae also cultured in BG-11 medium. Afterward the AgNPs were sonicated and added into Erle nmeyer which contains Chlorella vulgaris. The evaluation of chlorophyll and β-carotene content were done in the day 0, and 16. To get our favorable result the Camiel Eijckelhoff & Jan Dekker method were used, which is based on light absorption in 668.2 nm and 646.8 nm and 450 nm wavelength, by means of spectrophotometry device. The row results which have gained by spectrophotometry were analyzed by SPSS and Prism5 software. Our result shows that the effects of arginine coated AgNPs in the concentration of 100μg/mL has the most positive effect, and naked AgNPs in the concentration of 400μg/mL, most negative effect on chlorophyll content in chlorella vulgaris. The evaluation of β-carotene also have done by the same method in 450 nm wavelength, analyzed results on β-carotene content also shows the same results.
Abdolsamad s, Younes G, Yaghoobi MM
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